The song, Over There, written by George Cohan, was the most played on UK TV and radio advertising last year, according to the Performing Right Society.
That was followed by Go Compare’s rival Confused.com, which uses the Village People's YMCA in its advert.
In third place was the Lloyd's TSB adverts, which use the song Eliza's Aria by Elena Kats-Chernin.
Other songs that made the top 10 list include Speech Debelle's Spinnin for Sky, Blur's The Universal for British Gas and Take That's Shine for Morrisons supermarket chain.
PRS for Music said that in 2012 radio advertising accounted for £1.9m in broadcast royalties for its members, with television commercials netting £8m. Of all music played on UK commercial television stations, approximately one minute of every ten is used solely in adverts.
Dan Neale, Head of Music at advertising agency, Rainey Kelly Campbell Roalfe/Y&R said: "Music's role in TV adverts has always been significant, and under-rated, but it's never been more beneficial for songwriters to land that deal.
"The exposure and reach they can gain by getting a song in a commercial is a huge benefit to the artist, as it allows them to cut through the many thousands of acts that the modern audience has access to.
"The licence fee and royalties that are generated by the campaign can change a songwriter’s career."
Synchronisation, or sync, income from music used in ads is becoming an increasingly important revenue stream for artists.
Mercury Prize winner, Speech Debelle said: "Sync is an essential source of income for me so I can keep writing and producing music that makes people listen, and PRS for Music helps make that happen.”
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Advertising agencies can pay fees to secure the music they need to make their advert work. In addition to these initial sync fees, songwriters and publishers get paid royalties by PRS for Music every time the advert is broadcast on TV or radio and last year these totalled £10m.
PRS represents 95,000 songwriters, composers and publishers, tracking music used online, broadcast and performed live.