Narita International Airport, outside of Tokyo says JAL, reported a 100-litre fuel leak in a 787 during an inspection Sunday.
The aircraft reportedly was the same one that had a fuel leak in Boston on Tuesday, which saw its flight aborted after around 40 gallons of fuel spilled onto the runway.
On Monday a Japan Airlines-operated 787 Dreamliner caught fire after landing in Boston on a flight from Tokyo as the plane sat empty of passengers on the tarmac. It took firefighters 40 minutes to put out the blaze.
On Wednesday, Japan's All Nippon Airways said it cancelled a Boeing Dreamliner flight because of a brake problem, which was the third glitch to hit the next-generation aircraft in as many days.
Boeing has insisted that the 787's problems are no worse than what it experienced when its 777 was new in the mid-1990s.
The 787 is Boeing's newest and most high-tech airliner. Japanese airlines are among the top 787 customers.
On Friday, The US Federal Aviation Administration (FAA) announced it would conduct a comprehensive review of the design, manufacture and assembly of the Boeing 787 after recent incidents.
However, at the news conference in Washington, transportation officials said there was nothing in the data the agency had seen to suggest the plane was not safe to fly.
But US Transportation Secretary Ray LaHood said the agency wanted to figure out why the safety-related incidents are occurring.
The 787, which Boeing calls the Dreamliner, is now one of its top-sellers and is well-liked by airlines.
The plane is the aircraft maker's newest and most technologically advanced airliner.
In November 2010, a test flight had to make an emergency landing after an electrical fire. The incident delayed flight tests for several weeks while Boeing investigated.