The uncapped Paolo Odogwu and Harry Randall have been called up by England for the Six Nations.
Meanwhile, Owen Farrell will captain the side as expected.
Elliot Daly and Anthony Watson will both hope to receive their 50th caps during the course of the tournament, while Beno Obano could get his first.
England players will meet at St. George’s Park next Wednesday before moving to their Lensbury base in Teddington ahead of their Six Nations opener against Scotland at Twickenham on 6 February.
England will keep a 28-player squad throughout the competition following an agreement with Premiership Rugby, the RPA and the RFU due to challenges caused by coronavirus.
“This has been a really tough squad to pick and I know there are a lot of disappointed players who haven’t been selected,” head coach Jones said.
“We’re really excited by the players we have got, it’s a very vibrant squad and we are looking forward to getting started next week.
“We’re very grateful to Premiership Rugby, the clubs and the RPA for allowing us to have 28 players throughout the tournament, we’re very happy with the number and it’s testament to the growing relationships between all parties.
“Ultimately our goal is to win the Six Nations and to do that we’ll need to work hard, prepare well each day and take it as it comes.”
Jones also named a 12-player shadow squad, which will be adhere to the same testing protocols as the main squad.
Mako Vunipola will join the squad to undertake rehab during the tournament.
Following the Calcutta Cup against Scotland, defending champions England will host Italy on 13 February before travelling to Wales for their third match of the competition on 27 February.
England will then host France at Twickenham on 13 March before playing their final match of this year’s Six Nations in Dublin versus Ireland on 20 March.
Elliot Daly (Saracens) – 47 caps
Owen Farrell (Saracens) – 88 caps
George Ford (Leicester Tigers) – 72 caps
Ollie Lawrence (Worcester Warriors) – 3 caps
Max Malins (Bristol Bears) – 3 caps
Jonny May (Gloucester Rugby) – 61 caps
Paolo Odogwu (Wasps) – uncapped
Harry Randall (Bristol Bears) – uncapped
Dan Robson (Wasps) – 7 caps
Henry Slade (Exeter Chiefs) – 34 caps
Anthony Watson (Bath) – 46 caps
Ben Youngs (Leicester Tigers) – 104 caps
Luke Cowan-Dickie (Exeter Chiefs) – 26 caps
Tom Curry (Sale Sharks) – 28 caps
Ben Earl (Bristol Bears) – 8 caps
Ellis Genge (Leicester Tigers) – 23 caps
Jamie George (Saracens) – 54 caps
Jonny Hill (Exeter Chiefs) – 4 caps
Maro Itoje (Saracens) – 43 caps
Joe Launchbury (Wasps) – 69 caps
Courtney Lawes (Northampton Saints) – 85 caps
Joe Marler (Harlequins) – 72 caps
Beno Obano (Bath) – uncapped
Will Stuart (Bath) – 8 caps
Sam Underhill (Bath) – 22 caps
Billy Vunipola (Saracens) – 56 caps
Harry Williams (Exeter Chiefs) – 18 caps
Mark Wilson (Newcastle Falcons) – 19 caps
Charlie Atkinson (Wasps) – uncapped
Ali Crossdale (Saracens) – uncapped
Tom Dunn (Bath) – 3 caps
Charlie Ewels (Bath) – 17 caps
George Furbank (Northampton Saints) – 3 caps
Joe Heyes (Leicester Tigers) – uncapped
Jonathan Joseph (Bath) – 54 caps
Joe Marchant (Harlequins) – 4 caps
George Martin (Leicester Tigers) – uncapped
Alex Mitchell (Northampton Saints) – uncapped
Jacob Umaga (Wasps) – uncapped
Jack Willis (Wasps) – 2 caps