The versatile Arsenal left-back is one of his country’s most important players and would have been considered a guaranteed starter for Monday’s eagerly anticipated Group D contest on home soil at Hampden Park - Scotland’s first major tournament match for 23 years.
However, Tierney was a notable absentee from the team sheet revealed an hour before kick-off in Glasgow, with head coach Steve Clarke subsequently confirming that the former Celtic star was battling a minor, unspecified injury issue.
Scotland hope Tierney will still be fit in time for Friday’s showdown with old rivals England at Wembley.
Their final group fixture comes against Croatia - beaten 1-0 by England at the weekend - back at Hampden on Tuesday, June 22.
"Kieran picked up a little niggle in training during the week,” Clarke said before Scotland faced the Czechs.
“Not ready for this one, hopefully ready for the next one."